Juniper Publishers Gastroenterology Open Access Journals

  ADVANCED RESEARCH IN GASTROENTEROLOGY & HEPATOLOGY   JUNIPER PUBLISHERS Authored by Mohammad Firoz Alam Abstract The present study was designed to find out the hepatoprotective effect of Thymoquinone against cadmium induced liver toxicity in rat. Four different groups of rats were used for this study. In details Group-I was nornal rat; Group-II was toxic Cd (2 mg/kg/day) has given to rats subcutaneously for 7 days. Group-III was pre-treated with Thymoquinone and followed by Cd (2 mg/kg/day) for 7 days. Group-IV was given Thymoquinone (20 mg/kg/day) as a positive control for 7 days. At the end of experiment blood was taken for biochemical study (AST, ALT, ALP and Total Bilirubin) and thereafter animal was killed, and liver was dissected out to analyze oxidative stress (LPO, and GSH). The biochemical study indicated that the level of enzymes (AST, ALT, ALP and Total Bilirubin) was significantly (P≤0.01) elevated in the serum of Group-II and TBARS contents were also increas...

                          ADVANCED RESEARCH IN GASTROENTEROLOGY & HEPATOLOGY   JUNIPER PUBLISHERS Authored by Abdulghani Mohamed Alsamarai Abstract Background:  Genetic variations of the infectious agents play an important role on the influence of natural disease severity, response to treatment and morbidity. Aim:  To clarify if there are genetic variations in H. pylori clinical isolates from cases with gastritis, peptic ulcer and carcinoma of stomach. Materials and Methods: Eight PCR product sample were sent for sequence analysis of  H.pylori  isolated from biopsy of stomach and 20μl (10pmol) from the Forward primer sequence is 5′-GGTTCAAATCGGCTCACACT-3′ Reverse primer sequence is 5′-CTCCTTAATTGTTTTTACATAG-3′ They are made by primer 3PLUS software. The result of the sequence analysis was analyzed by blastn in the National Centre for Biotechnology Information (NCBI) and many software used to de...